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1 article(s) from Hölting, Kristin

Shift of the reaction equilibrium at high pressure in the continuous synthesis of neuraminic acid

Jannis A. Reich,
Miriam Aßmann,
Kristin Hölting,
Paul Bubenheim,
Jürgen Kuballa and
Andreas Liese

Beilstein J. Org. Chem. 2022, 18, 567–579, doi:10.3762/bjoc.18.59

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Graphical Abstract

Figure 1: Reaction sequence starting from GlcNAc with ManNAc as an intermediate. Pyr is added in the second s...

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Figure 2: Enzyme loading after immobilization of the epimerase and aldolase on different carriers.

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Figure 3: Evaluation of immobilized epimerase on different carriers with respect to specific activity. Reacti...

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Figure 4: Evaluation of immobilized aldolase on different carriers with respect to specific activity. Reactio...

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Figure 5: Relative activities in repetitive batch experiments of the immobilized epimerase on polymethacrylat...

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Figure 6: Relative activities in repetitive batch experiments of the immobilized aldolase on amino methacryla...

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Figure 7: Recycling study of immobilized epimerase and aldolase. Assay conditions: 100 mM Tris, pH 8, 40 °C, ...

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Figure 8: Measured reaction rates of the immobilized epimerase. The dashed line is the fit according to the M...

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Figure 9: Measured reaction rate of the immobilized epimerase as a function of pyruvate and pressure. Dashed ...

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Figure 10: Measured reaction rate (left) and the determined inhibition constant by pyruvate (right) at differe...

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Figure 11: Measured kinetics of the aldolase when varying the pyruvate and ManNAc concentration (given in mM) ...

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Figure 12: Circular reactor, vessel mixing was achieved with a magnetic stirrer and samples were taken directl...

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Figure 13: Aldolase: Change of the equilibrium constant at different pressures. Starting concentrations were v...

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Figure 14: Progress curve of the circular reactor with both reactions at varying pressures. Starting condition...

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Figure 15: Residence time distributions of the stand-alone system and the reactor integrated into the system. ...

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Figure 16: Reactor set-up (left to right): UHPLC pump, heated fixed-bed reactor, capillaries (ID: 25 µm or 50 ...

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Full Research Paper

Published 20 May 2022

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